The characters Essay Example for Free

The characters Essay Harry Burns: A complete slob and a brooding pessimist with a grim sense of humor. Sally Albright: Shes cheerful, organized, and optimistic. The set-up The two main characters, Harry Burns and Sally Albright, dislike each other immediately. Shes cheerful, organized, and optimistic. He is a complete slob and a brooding pessimist with a grim sense of humor. Not long after meeting, they are stuck with each other on a long car trip from Chicago to New York City. To pass the time, Harry asks Sally to tell him the story of her life. The scene: Harry: So†¦Ã¢â‚¬ ¦. since we have a long journey and I have all the time in the world. Tell me about your life. Your entire life story. Sally: My life story? Why would someone like you be interested in knowing my story? Harry: I am not interested. I just want to bite away some time. Sally: emm†¦ well, my life has nothing that interesting to talk about. Harry: I thought so†¦ u’r like one of those â€Å"high maintenance snooty new york execs† Sally: No, I’m not snooty! I am organized, I love my friends and care for my family. I don’t need to be judged by a slob like you. Harry: ohhhhhhh! Somebody’s offended. Sally: Ass holes like you think just because you can throw down a few drinks, and nail some slut at a swanky bar, your life must be wild! I despise people like you, mister! Harry: You despise us because you can never have what we have. Sally: We can have everything you have and much more. We just choose not to! Sally: I’m going to sleep now†¦ I can’t take any more of your noise! End of scene What does this exercise tell you about film dialogue and what makes it work most effectively? This exercise tells us that film dialogue needs to short. Not too long. It can be unexpected and doesn’t necessarily need to have logic. Does it suggest that dialogue in films is different from dialogue in literature and drama? Yes, I do believe dialogue in films is different from dialogue in literature and drama because in literature and drama the dialogues can be a little longer. But in films where each scene is about 2 minutes or less. It’s important to keep it short and sweet. In what ways is film dialogue close to or different from real-life conversation? Film dialogue is quite similar to real life conversation but unnecessary text may need to be cut out and all the lines would need to make an impact quickly to keep the audience interested. Since it needs to look real like the actors are actually having a conversation. It does have similar features of a real life conversation.

The Great Gatsby and the American Dream :: The Great Gatsby

Introduction F. Scott Fitzgerald’s novel, â€Å"The Great Gatsby†, is one of the few novels he wrote in 1925. The novel takes place during the 1920’s following the 1st World War. It is written about a young man named Nick, from the east he moved to the west to learn about the bond business. He ends up moving next to a mysterious man named Gatsby who ends up giving him the lesion of his life. After love circles with Gatsby and his cousin Daisy, lastly Jordan and gossip resulting with killings end up discussed over his experience resulting going back east. Through reading the book, it shows how the experience of selfness and World War 1 made the high social optimistic over their lives. It showed how they wanted to forget the past and build themselves back into their social class. Also, by the experience of Daisy and Jordan treatment from the men in the stories allows the reader to see how time still hasn’t changed how relationships work with men. This novel is a great novel to give an example on how reality is to people even the high class. Through the discussion of the passage, poem and scholarly article will show how the path towards the American Dream can turn into a negative or positive outcome in a person’s life. Theme When Mr. Fitzgerald wrote â€Å"The Great Gatsby,† he described the actions of the human society. In a certain way, not only did he describe and critic the high class but also the lower class, which ended up critiquing the American Dream. The American Dream was a idea give to believe that a human being should pursue being happy, wealthy, and loved which has cause any human being to go in search for this idea. All that is end up being found is the fact of having the illusion of having more material is to be happy than being happy by valuing what you already have. Gatsby would be a great example of not valuing his materials. He had everything he wanted – except Daisy of course – but he never used or appreciated the materials he was surrounded by. â€Å"You know, old sport, I’ve never used that pool all summer,† said Gatsby. He didn’t value anything but Daisy since the reason for everything he did was for her. It only leaves to question to whether he really loved her or wanted her wealth or maybe just even the accomplishment of having her.

Bacterial Transformation Lab Report Essay

Backround: The plasmid pGLO contains an antibiotic-resistance gene, ampR, and the GFP gene is regulated by the control region of the ara operon. Ampicillin is an antibiotic that kills E. coli, so if E. coli, so if E. coli cells contain the ampicillin-resistance gene, the cells can survive exposure to ampicillin since the ampicillin-resistance gene encodes an enzyme that inactivates the antibiotic. Thus, transformed E. coli cells containing ampicillin-resistance plasmids can easily be selected simply growing the bacteria in the presence of ampicillin-only the transformed cells survive. The ara control region regulates GFP expression by the addition of arabinose, so the GFP gene can be turned on and off by including or omitting arabinose from the culture medium. Purpose: The purpose of this lab was to understand bacterial transformation, how it occurs, and to make DNA glow. Hypothesis: If the transformed E. coli is mixed with the ampicillin resistance gene, it will be able to grow in the ampicillin plates, but the non-transformed E.coli will not. Materials: Two microcentrifuge tubes 500 uL of ice cold 0.05 CaCl2 E. coli bacteria A sterile plastic loop A sterile P-20 micropipette 10 uL of pAMP solution A timer Ice A water bath 500 uL of Luria broth A spreading rod Four plates Incubator Procedure: Day before lab 1. Streak E. coli host cells for isolation. 2. Prepare six source plates. Day of lab 1. Get two microcentrifuge tubes, which should each contain 200 uL of cold CaCl2 solution. Label one tube with your initials and a (+) and the other tube with your initials and a (-). 2. Transfer 2-4 large colonies using a sterile plastic loop to each microcentrifuge tube and completely resuspend. Do not transfer any agar. Put the tip of the loop into the CaCl2 solution and spin until there is not any cells on the loop. 3. Close each of the tubes and put them in ice. 4. Ask your teacher to use a P-20 micropipette to add pGLO DNA to your transformation mix. 5. Add pGLO DNA to the (+) labeled microcentrifuge tube. 6. Incubate both microcentrifuge tubes on ice for fifteen minutes. 7. Take both tubes out of ice and immediately place in incubator at 42Ù ¥C for 90 seconds. 8. After place both tubes back in the ice for two minutes. 9. Add 200uL Luria Recovery Broth to both microcentrifuge tubes. 10. Let both the tubes rest at room temperature for 10 minutes. 11. During the 10 minutes, get the LB agar and LB+AMP agar plates ready. Mark your plates with the transformation tube mixture to use (+ or -), the lab group names, and the date on the top of the dishes. 12. Add 100ul of the pGLO transformation cell mixture to the center of the agar surface of the corresponding LB agar and LB+AMP plates. 13. Use a sterile plastic loop to distribute the cell suspension evenly on the plate by â€Å"skating† the loop back and forth across the LB agar plate several times. 14. Use the same loop and technique to spread the same cell suspension (+) on the LB+AMP agar plates. Dispose of the sterile loop in a beaker of germicide. 15. Repeat the procedure by spreading the (-) transformation cell mixture to each of the (-) labeled LB and LB+AMP plates. Be sure to use a fresh plastic loop for the ‘None’ transformation mix. 16. Stack your group’s set of plates on top of one another and tape them together. The plates should be left upright position to allow the cell suspension to be absorbed by the agar. 17. Place the plates in an inverted position (agar side on top) in a 37Ù ¥C bacterial incubation oven for overnight incubation (15-20 hrs.). Day after lab 1. Lower the lighting in the room and use a long wave U.V. light to visualize the transformed cells that will glow due to the expression of the green or blue fluorescent proteins. Data: LB+ (Positive Control) LB- (Positive Control) LB/AMP+ (Experimental) LB/AMP- (Experimental) Bacterial Growth lawn lawn 3 colonies No growth Conclusions: The bacteria treated with the pAMP solution developed a resistance to ampicillin and were able to grow on the ampicillin plate. Those that were not treated with the pAMP were not able to grow on this medium. The plates with no ampicillin served as a control to show how the bacteria would look  in normal conditions. Transformation is never fully effective, Only cells that are competent enough are able to take up the foreign DNA. Therefore, the ampicillin+ plates showed less growth that the control plate. Questions: 1. Record your observations about the color and growth (number of colonies) of bacteria on the Petri plates. If you have so much bacterial growth that you can’t count individual colonies, this is referred to as â€Å"lawn.† LB+ (Positive Control) LB- (Positive Control) LB/AMP+ (Experimental) LB/AMP- (Experimental) Bacterial Growth lawn lawn 3 colonies no growth 2. Calculate the transformation efficiency of your transformation experiments. Transformation efficiency refers to the number of cells transformed per microgram (ug) of DNA. The transformation efficiency of my transformation experiments is 0.0125 cells transformed per microgram (ug) of DNA.

Dreams and Escape in The Glass Menagerie Essay - 2243 Words

Dreams and Escape in The Glass Menagerie None of the characters in The Glass Menagerie is capable of living in the present. Everyday life is so oppressive that each character, through their dreams, retreats into a fantasy world. This essay will examine the reality faced by Amanda, Tom, Laura and Jim and probe how, through their dreams, each character attempts to transcend reality. Amanda, having lost her husband and having to take care of her two children, namely Tom and Laura, who, in her eyes, are equally lost in their lives, leads a hard life. Having a son whom she considers unrealistic, daydreaming about becoming a recognized poet rather than staying committed to his present job, Amanda is not only overwhelmingly†¦show more content†¦On this occasion, she is dressed in the same girlish frock she wore on the day she met the childrens father, attempting to conceal her shabby present and recapture part of the elegance she associates with her giddy days of entertaining many gentleman callers. Bewildered by her immediate surroundings and unable to cope with the social and economic reality of the Depression days, Amanda is often obsessed with her past as the genteel southern belle dominated by refined social gatherings and elegant living conditions, reminiscing about her own experiences with men in Blue Mountain: One Sunday afternoon in Blue Mountain - your mother received - seventeen! - gentleman callers!... (Williams 16). Attempting to materialize her southern belle past, she even makes constant insistence on Lauras having gentleman callers. Tom, though not physically crippled as his sister Laura, finds himself paralyzed in the warehouse in which he works. Faced with the bleak aspects, and perhaps the bleak prospects, of the day-to-day factory job, he regards the warehouse as a prison that shackles all the basic impulses with which, he believes, men are endowed-Man is by instinct a lover, a hunter, a fighter (Williams 39). In the warehouse Tom cannot find any satisfaction at all - Id rather somebody picked up a crowbar and battered out myShow MoreRelated Dreams of Escape in The Glass Menagerie Essay example2299 Words   |  10 PagesDreams of Escape in The Glass Menagerie    Anyone can handle a crisis, but day-to-day living is the most trying aspect of life (Jackson 19). This is especially true in the drama The Glass Menagerie. 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In thisRead MoreAnalysis Of The Play The Glass Menagerie 1281 Words   |  6 PagesThe Glas Menagerie 4/29/15 Within the play The Glass Menagerie, Amanda, Laura, and Tom Wingfield all of have their own dreams that are continuously destroyed by the harshness of reality. Amanda, stuck in the ease of her youth, tries to relive her life through her daughter Laura. Being crippled both physically and mentally, Laura struggles to escape the bubble she has created around herself that her mother Amanda so strongly tries to force her out of. Tom whom, although reads poetry and dreams of escapeRead MoreEscape from Reality in The Glass Menagerie by Tennessee Williams680 Words   |  3 PagesEscape from Reality in The Glass Menagerie by Tennessee Williams The Characters Escape From Reality in The Glass Menagerie The Glass Menagerie by Tennessee Williams centers around a dream of escape. Although everyone wants to escape from a different reality, they all feel that need to get away. The father is the most successful in his escape because he never has to deal with anything at home. He actually leaves and doesnt look back. As for the other four: Laura, Amanda, Tom, and Jim, they seemRead More Essay on Stagnant Lives in Streetcar Named Desire and Glass Menagerie1196 Words   |  5 PagesStagnant Lives in Streetcar Named Desire and Glass Menagerie   Ã‚  Ã‚   The Stagnant Lives of Blanche DuBois and Amanda Wingfield  Ã‚  Ã‚   All of Williams significant characters are pathetic victims--of time, of their own passions, of immutable circumstance (Gantz 110). This assessment of Tennessee Williams plays proves true when one looks closely at the characters of Blanche DuBois in A Streetcar Named Desire and Amanda Wingfield in The Glass Menagerie. 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